The Luciferase Reporter Assay: How it works - Bitesize Bio

 

dual luciferase reporter assay

I.B. Format of the Dual-Luciferasefi Reporter Assay Quantitation of luminescent signal from each of the luciferase reporter enzymes may be performed immediately following lysate preparation without the need for dividing samples or performing additional treatments. The firefly luciferase. The luciferase reporter assay is commonly used as a tool to study gene expression at the transcriptional level. It is widely used because it is convenient, relatively inexpensive, and gives quantitative measurements instantaneously. It has broad applications across various fields of cell and Author: Farida Khan. The Dual-Luciferase® Reporter (DLR™) Assay System provides an efficient means of performing two reporter assays. In the DLR™ Assay, the activities of firefly and Renilla luciferases are measured sequentially from a single sample.


Dual Luciferase Assay System for Rapid Assessment of Gene Expression in Saccharomyces cerevisiae


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Catalog number selected: E This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs. Both assays can be completed in about 4 seconds using a luminometer with reagent auto-injectors. Notice for Cat. For applications requiring more lysis reagent e.

See all certificates for E No results, dual luciferase reporter assay. Consecutive detection of firefly and Renilla luciferase activities in a single sample. Two-hour signal half-life. No injectors required. A sensitive reporter assay for continuous-processing on robotic systems. Enhanced performance in a range of cell types, including difficult-to-transfect cell lines.

High-performance microplate reader for detecting luminescence, fluorescence and absorbance. Our website does not fully support your browser, dual luciferase reporter assay. Your Account Username Account not found. Email address dual luciferase reporter assay unverified. Account is locked. Password Incorrect password. Password reset is required. Account is invalid.

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dual luciferase reporter assay

 

I.B. Format of the Dual-Luciferasefi Reporter Assay Quantitation of luminescent signal from each of the luciferase reporter enzymes may be performed immediately following lysate preparation without the need for dividing samples or performing additional treatments. The firefly luciferase. The luciferase reporter assay is commonly used as a tool to study gene expression at the transcriptional level. It is widely used because it is convenient, relatively inexpensive, and gives quantitative measurements instantaneously. It has broad applications across various fields of cell and Author: Farida Khan. Jun 02,  · Dual luciferase assays. Dual luciferase assays were performed with the dual luciferase reporter assay system (Promega Corp., Madison, WI). All reagents were prepared as described by the manufacturer. The 5× passive lysis buffer was supplied by the manufacturer and used for cell lysis as described below in eltaini.ml by: