Enzymes: Properties and Mechanism of Enzyme Action

 

properties of enzymes lab report

Enzymes. The Rate of Reaction on Liver, Apples, and Potatoes with Hydrogen Peroxide. AP Biology, Mod 5. Abstract The purpose of this lab was to test enzyme function in various environments. Substrate concentration, temperature, and pH all affect the chemical reaction. Enzymes contain a cavity called the active site, to which the substrate, the substance whose reaction is catalyzed by the enzyme, bonds, forming the enzyme-substrate complex. Only one type of substrate can bond with the enzyme, in a lock-and-key manner. The enzyme then breaks the substrate down to form the products of the organic reaction. Enzyme Catalysis Lab Report Pre-lab Questions: 1. Write a balanced chemical equation with state symbols for the reaction catalyzed by peroxidase. 2H 2O 2 2H 2O + O 2 (4H 1 4O) (4H + 2O + 2O) 2. What is the substrate(s) of this reaction? What is the catalyst? Substrate = H 2O 2 hydrogen peroxide Catalyst = peroxide 3.


Enzymes lab report - Get Top Grade With Perfect Essay From eltaini.ml


This preview shows page 1 - 3 out of 9 pages. Subscribe to view the full document. I cannot even describe how much Course Hero helped me this summer. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero. University of Southern California. BISC Uploaded By esarver. Chapter 2: Properties of Enzymes Purpose: The purpose of the chapter 2 lab was to determine a variety of enzymatic properties.

Enzymes are large proteins which act as catalysts in many biological reactions. An enzyme is composed of one or more polypeptide chains, which are uniquely folded into an elaborate 3 dimensional shape, properties of enzymes lab report.

The substrate fits onto the enzyme in a manner, which is comparable to a lock and key. If it is not folded correctly, the substrate will not be able to bind to the reaction site, and therefore the enzyme would be rendered useless, unable to catalyze a reaction. The enzyme that we used in our experiments was Peroxidase, a large protein containing several hundred amino acids with an iron atom located at its active site.

Peroxidase normally functions to convert H 2 O 2 into water and O 2. We performed tests on the peroxidase with dye-coupled reactions. These properties of enzymes lab report are the O 2 from the break down of H 2 O 2 reacting with a dye added to solution. Once the gauiacol and the solution were mixed. The purpose of this lab was to find out properties of enzymes lab report what conditions Peroxidase reacts with H 2 O 2.

Share this link with a friend: Copied! Other Related Materials 10 pages.

 

Liver Enzyme Lab Report

 

properties of enzymes lab report

 

Enzymes Lab Report Refer to the section of the lab manual called ╩║How to Write a Lab Report╩║ in the appendix for general guidance on how to write the report. It must be typed. Be sure to address the issues highlighted in the lab manual and by your instructor in the. Sometimes, different enzymes may act on the same substrate to produce different end products. The substrate specificity of enzyme is based on amino acids sequence in the catalytic site as well as the optical isomeric form of the substrate. 4. Catalytic properties. Enzymes. The Rate of Reaction on Liver, Apples, and Potatoes with Hydrogen Peroxide. AP Biology, Mod 5. Abstract The purpose of this lab was to test enzyme function in various environments. Substrate concentration, temperature, and pH all affect the chemical reaction.